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产品型号： CRL-2873
产品代码：
产品价格：
计量单位：2500
折 扣 率： 0
最后更新：2014-08-07
关 注 度：3066
生产企业：上海迪奥生物科技有限公司
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产品详细介绍ATCC® Number:
CRL-2873™ Price:
Designations: HUVEC-CS Depositors: IM Bird
Biosafety Level: 1 Shipped: frozen
Medium & Serum: See Propagation Growth Properties: adherent
Organism: Homo sapiens (human) Morphology: endothelial
Source: Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Isolation: Isolation date: August, 1998
Age: newborn
Comments: HUVEC-CS was derived from HUV-EC-C (ATCC CRL-1730?) by initially passaging in tissue culture plastic flasks without additional growth factor like ECGS. Subsequently the HUVEC-CS cell line was plated on gelatin-coated flasks. It exhibits positive acetylated low-density lipoprotein (AcLDL) uptake and expresses eNOS, CD31 and ve-cadherin (classical markers of endothelial cells).It spontaneously forms capillary-like structures when grown on Matrigel. Receptors were detected for angiotensin II (AII), bradykinin and ATP.[PubMed: 15350190]. Distribution freeze lots have a doubling potential of 5 to 8 PDLs.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20.
Temperature: 37.0°C
Growth Conditions: Use gelatin-coated flasks.
Subculturing: Protocol: Volumes used in this protocol are for 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.05 (w/v) Trypsin- 0.53 mM EDTA solution (GIBCO Cat# 25300-054) to remove all traces of serum that contains trypsin inhibitor.3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to10 minutesDiscard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new porcine gelatin coated culture vessels. An inoculum of 1X 10(4) to 2X 10(4) viable cells/cm2 is recommended. Place culture vessels in incubators at 37°C.
Subcultivation Ratio: 1: 3 to 1: 4
Preservation: Freeze medium: Culture medium,90; DMSO, 10
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 36 hours
Related Products: source culture:ATCC CRL-1730
References: 91856: Gifford SM, et al. Functional characterization of HUVEC-CS: Ca2+ signaling, ERK 1/2 activation, mitogenesis and vasodilator production. J. Endocrinol. 182: 485-499, 2004. PubMed: 15350190
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