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产品型号： F500
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最后更新：2014-08-07
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生产企业：上海迪奥生物科技有限公司
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产品详细介绍Infinite® F500 多功能高通量酶标仪
Infinite® F500 - Overview
An infinite relationship with your assays
The Infinite F500 is Tecan’s most sensitive filter-based multimode microplate reader, to provide a multifunctional and modular system which offers excellent levels of sensitivity for fluorescence- and luminescence-based assays. The Infinite F500 is compatible with a wide variety of microplates, from 6- to 1,536-wells, including standard and low volume plate formats and Tecan’s patent pending NanoQuant Plate™. Its high speed, on-the-fly measurement capabilities ensure maximum throughput, allowing complete reading of a 1,536-well plate in under 30 seconds. It supports a broad range of measurement modes and functions, allowing straightforward application of many current assay technologies, and its modular design ensures your instrument can grow with your needs.
Infinite® F500 - Features
High measurement speed and robotic compatibility
The Infinite F500 filter-based microplate reader is capable of reading 6 to 384-well plates, including standard, half-area and low volume 384-well plates. All top-reading modes can be used for 1536-well microplates (optional) and, by selecting the high speed »on-the-fly« mode, a 1536-well plate can be read in less than 30 seconds (please refer to typical performance values). The Infinite F500 can also be easily integrated into the Freedom EVO® series of automated platforms.
NanoQuant Plate™ compatibility: DNA quantification in 2 μl samples
The patent pending NanoQuant Plate allows the measurement of up to 16 samples simultaneously, in volumes as low as 2 μl. This 16 individual quartz optics are a great solution for DNA/RNA quantification, as well as quality control applications, and are compatible with multichannel pipettes for easy sample application.
NanoQuant Plate for Infinite F500 plate reader for DNA quantification
z-Optimization: for better results in low volumes
The Infinite F500 microplate reader allows you to automatically adjust the z-focus in all top-reading modes (fluorescence intensity and polarization, luminescence), significantly improving the quality of measurements. The automated z-focusing makes it easy to optimize the set-up and adapt the instrument settings to highly demanding assay parameters, such as low volumes or different well shapes.
Infinite F500 plate reader with automated z-Optimization
Programmable filter slides
The Infinite F500 filter-based microplate reader includes programmable filter slides with electronic chips; a great tool for saving certain filter characteristics to support the filter definition of the corresponding filter
1 The mirror carrier can be extended with a custom dichroic mirror; 2 Exchangeable filters can be easily fitted into the excitation and emission filter slides; 3 Separate excitation and emission filter slides with 6 filter positions each ; 4 Microchip on filter slide to facilitate filter definition/recognition; 5 Filter assembly tool
Infinite® F500 - Applications
The optimal filter based microplate reader for biopharmaceutical, life science and research laboratories
The fast and sensitive Infinite F500 filter-based microplate reader is ideal for a broad range of applications, in particular ratiometric assays and other technologies routinely used within assay development, screening and ADMET laboratories, research institutes and the Life Sciences industry. It is capable of reading 6 to 1536-well microplates, including standard and low-volume plates.
The Infinite F500 microplate reader supports many different applications and assay technologies, such as:
TR-FRET-based assays: e.g. HTRF® (Cisbio bioassays, France), LanthaScreen® (Invitrogen). FRET-based assays: e.g. Z`-LYTE® (Invitrogen) to study cellular protein kinases, GeneBLAzer® (Invitrogen) Reporter Gene assays. Glow luminescence: cell-based & reporter gene assays Flash luminescence: e.g. dual luciferase assay (DLR®) and various CA2+ detection assays if optional injectors are installed Fluorescence polarization: e.g. Predictor™ hERG Fluorescence Polarization Assay (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Red (Invitrogen), PolarScreen™ Glucocorticoid Receptor Competitor Assay Kit, Green (Invitrogen), Transcreener® ADP Assay (BellBrook Labs)
The Infinite F500 has been validated by Invitrogen for the following Invitrogen assays:
LanthaScreen® Adapta® Universal Kinase Assay Omnia® Z’-LYTE® GeneBLAzer® PolarScreen™ Predictor™ hERG Fluorescence Polarization Assay
If you are interested in more details on the applications for the Infinite F500 microplate reader, please take a look at the related literature.
The Infinite F500 has been granted the LanthaScreen® Certified Plus Status by Invitrogen
PolarScreen and Predictor are trademarks of Invitrogen Corporation, Carlsbad, USA.
LanthaScreen, Adapta, GeneBLAzer, Omnia and Z’-LYTE are registered trademarks of Invitrogen Corporation, Carlsbad, USA.
The Infinite F500 has been successfully validated and certified for HTRF® by CisBio bioassays.
HTRF® is a registered trademark of Cisbio international, France. The fluorescence ratio associated with the HTRF® readout is a correction method developed by Cisbio international and covered by the US patent 5,527,684 and its foreign equivalents, for which Cisbio international has granted a license to Tecan Group Ltd. Its application is strictly limited to the use of HTRF® reagents and technology, excluding any other TR-FRET technologies.
The Infinite F500 has been successfully Transcreener® Far Red FP and Transcreener® FI validated by BellBrook Labs, LLC, USA and meets the requirements for BellBrook Labs’ Instrument Validation Program.
Transcreener® HTS Assay Platform is a patented technology of BellBrook Labs, LLC USA. Transcreener® is a registered trademark of BellBrook Labs, LLC USA.
The Infinite F500 has passed the DLReady™ validation at Promega and is certified as DLReady™.
DLReady™ is a trademark of Promega Corporation, USA
Infinite® F500 - Typical performance values
Light sources
High energy xenon flash lamp
Wavelength selection
Filter-based
Wavelength range
Absorbance 230 – 1,000 nm
Fluorescence Ex: 230 – 900 nm; Em: 280 – 900 nm
Fluorescence polarization Ex: 300 – 750 nm; Em: 330 – 750 nm
Detectors
Absorbance UV Silicon photodiode
Fluorescence Extended wavelength (UV and far red) ;
Low dark current PMT
Luminescence Photon counting; Low dark current PMT
Plate formats
6 – 1,536-well plates, NanoQuant Plate
Temperature control
Ambient +4 to 42 °C
Shaking
Linear, orbital with variable amplitudes
Fluorescence sensitivity values
Fluorescence top 10 amol/well fluorescein
(Greiner® 384-well low volume black plate; 10 μl)
Fluorescence bottom 0.4 fmol/well fluorescein
(Greiner 96-well SensoPlateTM; 200 μl)
TRF 0.5 amol/well europium
(Greiner 384-well low volume white plate; 10 μl)
FP < 2 mP standard deviation @ 1 nM fluorescein
Luminescence sensitivity values
Glow luminescence* 225 amol/well ATP
(Greiner 384-well low volume white plate; 25 μl)
Flash luminescence** 12 amol/well ATP
(Greiner 384-well white plate; 55 μl)
Absorbance
Measurement range 0 – 4 OD
OD accuracy 0 – 2 OD: ≤ ± (1 % + 10 mOD)
OD reproducibility 0 – 2 OD: ≤ ± (0.5 % + 5 mOD)
Fastest reading times (on-the-fly)
Fluorescence (kinetic cycle) 1,536-well: 26 sec 384-well: 19 sec
Fluorescence (plate in/out) 1,536-well: 40 sec 384-well: 35 sec
Injectors
Injection volume Variable; 1 μl increments
Syringe size Selectable: 500 / 1,000 / 2,500 μl
Pump speed 100 – 300 μl/sec
Dead volume 100 μl (including pump back)
Accessories
Stacker Connect
Barcode scanner left or right
* 144-041 ATP detection kit SL (Biothema)
** ENLITEN® ATP assay system (Promega)
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